Leaf carbohydrates assimilation and metabolism affect seed yield of rapeseed with different waterlogging tolerance under the interactive effects of nitrogen and waterlogging

Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties.     

DPC~+化学封顶对不同施氮量下棉花叶片光合生理特性的影响

在北疆气候条件下,为明确棉花对不同施氮量下增效缩节胺[DPC+,25%甲哌鎓(1,1-dimethylpiperidinium chloride, DPC)水剂]化学封顶效应的响应,以新陆早53号为试验材料,在150(N1)、300(N2)和450(N3) kg hm~(–2)施氮水平下,以人工打顶(P0)为对照,研究DPC+剂量[450(P1)、750(P2)和1050(P3) mL hm~(–2)]对棉花叶绿素含量(Chl)、叶面积(LA)、气体交换参数、叶绿素荧光参数及干物质累积与分配的影响。结果表明,同一DPC+剂量下,随施氮量增加, Chl、LA、气体交换参数、叶绿素荧光参数和生殖器官干物质(RODM)均呈现先上升后下降的趋势;同一施氮量下,上述参数因DPC+剂量不同呈不同变化趋势,其中N1水平下以P1处理、N2水平下以P2处理、N3水平下以P3处理表现出较高的RODM、Chl、净光合速率(Pn)、实际光化学效率(ФPSII)、电子传递速率(ETR),较低的非光化学淬灭(NPQ)。DPC+与施氮量互作表现为,与其他处理相比, Chl、Pn、蒸腾速率(Tr)、气孔导度(Gs)、ФPSII、ETR、RODM在N2P2处理下分别提高了15.52%、29.39%、27.97%、36.77%、23.28%、23.55%、8.41%～22.24%, NPQ降低了34.54%。相关分析表明,干物质累积和Chl、LA、Pn、Gs、ФPSII均呈显著正相关,与NPQ呈极显著负相关。因此,在喷施DPC+(750 mL hm~(–2))条件下,适量追施氮肥(300kghm~(–2))能改善棉花光合性能,在增加干物质累积的基础上,促进了光合产物向生殖器官分配。     

春大豆鼓粒期冠层翻叶原因及翻叶对叶片净光合速率和粒重的影响

为明确春大豆鼓粒期冠层翻叶原因及翻叶对粒重的影响。在大田条件下,以28份大豆品种为材料,采用随机区组试验设计,研究了鼓粒初期植株顶部4片叶的单叶面积、比叶重、叶型指数、叶片含水量与翻叶率的关系;比较了翻叶与对照叶片的净光合速率、百粒重及粒重的差异。结果表明,不同大豆品种间翻叶率在0~26.5%之间,且差异显著,其中以‘中黄313’、‘中黄42’和‘中黄13’最高;单叶面积在46~151 cm²之间,其中以‘中黄39’、‘中黄42’和‘中黄13’最大;比叶重在0.536~1.64 mg/cm²之间,其中以‘中黄322’、‘中黄313’和‘中黄13’最低;叶型指数在1.35~3.51之间,其中以‘中黄70’、‘中黄80’和‘中黄42’最低。供试大豆品种分为3个类群,第I类群品种单叶面积小,比叶重高,叶型指数大,翻叶率为1.03%;第III类群品种单叶面积大,比叶重小,翻叶率高达19.7%;第II类群共4个品种,单叶面积、比叶重、叶型指数、翻叶率介于第I类和第III类群之间。翻叶后显著降低叶片净光合速率,平均降幅31.7%,粒数较多的‘新大豆27号’百粒重降低10.38%~22.4%,而粒数少的‘吉育60’粒重没有降低。大豆上层叶片单叶面积大、比叶重小及叶型指数低的叶片翻叶率高,翻叶降低叶片净光合速率,进而降低同节位百粒重及单节粒重,不利于大豆高产。     

异速对辊式玉米秸秆粉碎还田装置设计与试验

针对玉米秸秆量大、韧性强,导致还田后秸秆粉碎不均匀影响后续整地和播种等问题,该研究提出了一种异速对辊及动态双支撑形式的玉米秸秆粉碎还田方式,并研制了相应的玉米秸秆还田装置,主要由捡拾粉碎单元、对辊滑切支撑单元、支撑板和机壳等组成。在异速动态双支撑条件下,通过对作业过程中玉米秸秆动力学分析和秸秆漏捡面积分析,对捡拾粉碎刀和对辊滑切支撑刀进行设计,建立了影响秸秆粉碎合格率的数学模型。以捡拾粉碎刀转速、L型甩刀折弯角、L型甩刀刃口长度和滑切支撑刀滑切角为因素,秸秆粉碎合格率为试验指标进行Box-Behnken试验。田间试验结果表明:当滑切支撑刀滑切角45°、捡拾粉碎刀转速1 700 r/min、L型甩刀刃口长度45 mm和L型甩刀折弯角40°时,玉米秸秆粉碎合格率为92.58%,与预测值误差5%,满足国家标准要求。该研究提出的秸秆粉碎还田方式和研制的秸秆粉碎装置为玉米秸秆粉碎还田机设计和优化提供新的方案和技术支撑。     

The roles of light and pericarp on seed dormancy and germination in feral Raphanus sativus (Brassicaceae)

The timing of seed germination may determine the success of a weed species in an agroecosystem, and its expression is modulated by environmental conditions, but also by seed physiology and anatomy. The aims of this study were to investigate the roles of light, pericarp, dry storage and cold stratification on seed dormancy and germination in feral radish, a troublesome agricultural weed in temperate zones of the Americas that reduces crop yields. To this end, we used isolated intact pods and extracted seeds to test germination over time under contrasting temperature, light and storage conditions. Here, we showed that fresh seeds were non‐dormant, but that light and the presence of the pericarp reduced germination, especially under low temperatures. The pericarp reduced the final water content absorbed by seeds inside pods and decreased absorption/dehydration rates. The pericarp showed several small lignified cell layers in the endocarp, and x‐ray images displayed the lack of space between the partially embedded seed and the endocarp. Dry storage and cold stratification were ineffective in breaking the dormancy imposed by the pericarp. The apparent requirement for darkness and the mechanical restriction of the pericarp may have the potential to induce dormancy, spreading the timing of seed germination over a more extended period and hindering the control of feral radish.     

陕西周至县猕猴桃的运输损坏研究

通过分析陕西猕猴桃自身的生理特性,并结合陕西周至县猕猴桃的包装形式及运输环境,找出猕猴桃运输损坏的原因,提出猕猴桃合理的运输方案,为快递企业生鲜水果的运输方式提供一定的理论参考。     

Interactive effects of family and stocking density on survival and growth of the sea urchin Strongylocentrotus intermedius

To investigate the effects of family, stocking density, and their interaction on mortality, growth rate, and size dispersal in the sea urchin Strongylocentrotus intermedius, 1,440 juveniles from six full‐sib families were exposed to three stocking densities, that is, low stocking density (LD), middle stocking density (MD), and high stocking density (HD), and reared for 10 months in sea‐based suspended cages. The results demonstrated significant family effects on cumulative mortality rate (CMR); specific growth rate for test diameter (SGR for TD); specific growth rate for body weight (SGR for BW); coefficient of variation for body weight at the 10th month (CV for BW₁₀); and significant stocking density effects on SGR for TD, SGR for BW, and CV for BW₁₀. Statistically significant family by stocking density interaction was only found in SGR for TD. A certain degree of family‐ranking inversions occurred in SGR for TD. The present study provides evidence for the existence of family by stocking density interaction on the growth rate of test diameter in the family selection for S. intermedius. More attention should be paid to this interaction effect to select correct parents in S. intermedius.     

不同还田方式下北方旱作小麦秸秆腐解规律研究

为揭示不同还田方式下北方旱作小麦秸秆腐解规律,以小麦秸秆为试验材料,采用尼龙网袋法,于2014-2015年度在山西临汾保护性耕作长期定位试验区进行试验,设置秸秆还田旋耕(SRT)、秸秆覆盖免耕(SNT)2个处理,分析不同耕作方式下小麦秸秆的纤维素、半纤维素、木质素、有机碳、全氮含量以及秸秆腐解率、有机碳矿化率、全氮释放率的变化。结果显示,经过375 d的田间腐解,SRT秸秆腐解率为50%,SNT秸秆腐解率为31%。SRT秸秆的纤维素、半纤维素、木质素的平均腐解率分别为65%、45%、53%,SNT分别为56%、30%、39%;SRT秸秆有机碳的矿化率为57%,SNT为40%;SRT秸秆全氮素释放率为15%,SNT为36%。研究表明,SRT可加快秸秆腐解与组成成分的分解,SNT有利于秸秆氮素的释放,可减少农田氮投入。SNT秸秆有机碳矿化率低可减少农田碳循环速率,提高麦田固碳能力。本研究结果对我国北方旱作小麦秸秆还田管理具有一定的借鉴参考价值。     

秸秆还田配施腐熟剂对砂性土壤性质及滴灌玉米生长的影响

为探究秸秆还田配施腐熟剂对宁夏扬黄灌区土壤改良和玉米增产的效应,在秸秆还田条件下施用3种不同腐熟剂[生物秸秆速腐剂(SR+BS)、EM菌秸秆腐熟剂(SR+RJ)、有机废物发酵菌曲(SR+OW)],以秸秆还田不施腐熟剂处理为对照(CK),研究其对秸秆生物失重率、砂性土壤理化性状和滴灌玉米生长及产量的影响。结果表明,3种腐熟剂均能有效促进玉米秸秆腐解,其中SR+RJ的秸秆腐解程度最佳,翻埋130 d后其秸秆生物失重率为49.9%,SR+OW和SR+BS次之,3种处理分别较CK显著提高7.1、5.7、5.2个百分点。SR+RJ对改善0~40 cm土层土壤容重效果最佳,较CK显著降低4.2%,同时显著提高了耕层土壤有机质和速效养分含量及生育中后期土壤贮水量,SR+OW和SR+BS次之。施用秸秆腐熟剂能明显促进玉米生育中后期植株生长,其中SR+RJ最佳。SR+RJ和SR+OW对玉米增产增收效果最明显,分别较CK显著增产26.9%、23.4%,显著增收28.8%、23.4%。可见,秸秆还田配施腐熟剂可有效促进玉米秸秆腐解,改善土壤理化性质,促进玉米生长发育,显著提高作物产量与经济效益,以EM菌秸秆腐熟剂效果最佳。本研究为宁夏扬黄灌区还田后促进秸秆腐熟和砂性土壤培肥及玉米高产提供了技术参考。     

60Co-γ射线对睡莲生物学效应的影响

为探究辐射对睡莲的生物学效应,对2个睡莲品种弗吉尼亚(Nymphaea Virginia)和奥毛斯特(N.Almost black)的块茎进行不同剂量的⁶⁰Co-γ射线辐射处理。结果表明,2个睡莲品种的存活率均随辐射剂量的增加而下降,半致死剂量分别为24.342和27.671 Gy。5~10 Gy 剂量⁶⁰Co-γ辐射处理使睡莲叶面积和浮叶数显著增加,20~40 Gy剂量⁶⁰Co-γ辐射处理使叶面积和浮叶数显著减少。2个睡莲品种的开花时间均随辐射剂量的增加而延迟,但整个花期长度无显著变化。10~40 Gy剂量⁶⁰Co-γ辐射处理显著降低了睡莲花径,但对睡莲开花率无显著影响。辐射处理导致2个睡莲品种的叶片均出现红棕色斑块、锯齿、皱缩、小孔、卷曲、黄化等变异,且红棕色斑块的面积随着辐射剂量的增加而增大。辐射处理使黑红色花品种奥毛斯特出现普遍的褪色现象,而白色花品种弗吉尼亚的花色未发生变异。此外,经⁶⁰Co-γ射线辐射处理后2个睡莲品种均出现了花型变异的现象。本研究结果为利用辐射诱变技术选育睡莲新品种奠定了基础。